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Integrator, a Multiprotein Mediator of Small Nuclear RNA Processing, Associates with the C-Terminal Repeat of RNA Polymerase II

The C-terminal domain (CTD) of RNA polymerase II (RNAPII) is an essential component of transcriptional
regulation and RNA processing of protein-coding genes. A large body of data also implicates the CTD in the transcription and processing of RNAPII-mediated small nuclear RNAs (snRNAs). However, the identity
of the complex (or complexes) that associates with the CTD and mediates the processing of snRNAs has
remained elusive. Here, we describe an RNA polymerase II complex that contains at least 12 novel subunits,
termed the Integrator, in addition to core RNAPII subunits. Two of the Integrator subunits display
similarities to the subunits of the cleavage and polyadenylation specificity factor (CPSF) complex.
We show that Integrator is recruited to the U1 and U2 snRNA genes and mediates the snRNAs’ 3 end
processing. The Integrator complex is evolutionarily conserved in metazoans and directly interacts with the C-terminal domain of the RNA polymerase II largest subunit.

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Date Of Record Release 2009-09-03 16:51:30
Description The C-terminal domain (CTD) of RNA polymerase II (RNAPII) is an essential component of transcriptional
regulation and RNA processing of protein-coding genes. A large body of data also implicates the CTD in the transcription and processing of RNAPII-mediated small nuclear RNAs (snRNAs). However, the identity
of the complex (or complexes) that associates with the CTD and mediates the processing of snRNAs has
remained elusive. Here, we describe an RNA polymerase II complex that contains at least 12 novel subunits,
termed the Integrator, in addition to core RNAPII subunits. Two of the Integrator subunits display
similarities to the subunits of the cleavage and polyadenylation specificity factor (CPSF) complex.
We show that Integrator is recruited to the U1 and U2 snRNA genes and mediates the snRNAs’ 3 end
processing. The Integrator complex is evolutionarily conserved in metazoans and directly interacts with the C-terminal domain of the RNA polymerase II largest subunit.
Classification
Resource Type
Format
Subject
Keyword RNA, Genetics, Research
Date Of Record Creation 2009-09-03 16:44:30
Education Level
Date Last Modified 2010-09-23 18:32:23
Language English
Date Record Checked: 2009-09-03 00:00:00 (W3C-DTF)

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